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(1993) Free radicals as mediators of tissue injury and disease Critical Reviews in Toxicology, 23, 21 48 Keller, G, TG Warner, KS Steimer & RA Halewell (1991) Cu-Zn superoxide dismutase is a peroxisomal enzyme in human broblasts and hepatoma cells Proceedings of the National Academy of Sciences of the USA, 88, 7381 7385 Kille, P, J Kay & GE Sweeney (1993) Analysis of regulatory elements anking metallothionein genes in Cd-tolerant sh (pike and stone loach) Biochimica et Biophysica Acta, 1216, 55 64 Kling, P & P-E Olsson (1995) Regulation of the rainbow trout metallothionein-A gene Marine Environmental Research, 39, 117 120 Kling, P, LJ Erkell, P Kille & P-E Olsson (1996) Metallothionein induction in rainbow trout gonadal (RTG-2) cells during free radical exposure Marine Environmental Research, 42, 33 36..

Reduced levels of blood cortisol and thyroxin have been reported in several species of sh chronically exposed to mixtures of pollutants including heavy metals, PAHs, PCBs and bleached craft mill ef uent Hontela et al (1995) reported lower levels of blood cortisol and thyroxin in sexually immature and mature male and female yellow perch (Perca avescens), from a site contaminated by organic and heavy metal contaminants compared to sh from a reference site In addition, the contaminated sh exhibited greater liver glycogen stores and had smaller gonads and lower condition factor than sh from the clean site This endocrine impairment, characterised by a reduced ability to elevate plasma cortisol in response to stress, has also been described in northern pike (Esox lucius) from contaminated sites In each case the sh showed reduced ability to respond to adrenocorticotropic hormone (ACTH), indicating disruption to the normal neuroendocrine response (Hontela, 1998).

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24 As powerful and utilitarian as Putnam believes these attributes to be, and as inherent in the American psyche as de Tocqueville found them almost two centuries ago, they may now be waning Putnam cites a broad range of research and statistics that suggests that Americans are not as civic as they once were, or at least are not so in the traditional manner He notes, for example, that membership in a wide variety of organizations, from the American Association of University Women to the Parent-Teacher Association and from B Nai B rith to the American Bar Association are down significantly Most of these groups tended to peak around the middle of the century, roughly from the early 1950s through the late 1970s.

It is suggested that lifelong exposure to chemical pollutants may lead to an exhaustion of the cortisol producing endocrine system, possibly as a result of prolonged hyperactivity of the system (Hontela et al, 1992) Catecholamines have been reported to be released in response to conditions which give rise to hypoxaemia (Thomas & Perry, 1992) This may have a number of bene ts including stimulation of splenic release of erythrocytes to aid oxygen carrying capacity (Pearson et al, 1992; Alkindi et al, 1996) However, high catecholamine levels as well as structural damage to the gill are also prime causal factors for induced systemic hydromineral disturbance (Bonga, 1997) This is associated with increased cellular turnover in these organs In sh, cortisol combines glucocorticoid and mineralocorticoid actions, with the latter being essential for the restoration of hydromineral homeostasis.

An inability to raise blood cortisol levels may therefore indicate a breakdown in this homeostatic mechanism Two opposite behavioural coping strategies to stress appear to be associated with this neuroendocrine mechanism Van Raaij et al (1996) subjected rainbow trout to severe hypoxia and measured blood levels of catecholamines, cortisol, glucose, FFA, lactate and electrolytes Approximately 60% of the sh survived the experiment Behavioural strategy appeared to be highly related to survival Non-surviving sh displayed strenuous avoidance behaviour whereas surviving sh did not panic and remained quiet Behavioural differences were associated with marked differences in plasma catecholamine levels which were four to ve times higher in non-surviving sh The cortisol response tended to be lower in the nonsurviving sh There is also growing supporting evidence for interaction between the neuroendocrine system and the immune system in sh.

25 At the same time, he cites research that indicates that in those states with higher relative levels of social capital there are impressive benefits to be realized: kids are better off, by any number of measures; schools work better; kids watch less television; violent crime is rarer; mortality is lower; and health is better26 In thinking about these figures, Putnam speculates that perhaps they represent the influence of four primary factors First, he notes, is the pressure of time and money, most frequently thought of as the two-income household that has little energy for other involvements Second, he believes, is commuting and the sprawl of the suburbs, which have pulled us further apart spatially Third, he opines, is the impact of the various forms of electronic entertainment that allow us to do alone what previously we would have done in groups.

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